|本期目录/Table of Contents|

 一组分离自男男性行为人群HIV-1毒株的近似全长序列分析(/HTML)

《中国病毒病杂志》[ISSN:2095-0136/CN:11-5969/R]

期数:
2011年02期
页码:
99-105
栏目:
论著
出版日期:
2011-04-01

文章信息/Info

Title:
 Analysis of a panel of HIV-1 near-full length sequences from MSM
作者:
 

王铮庄道民刘思扬陈立力李林刘永健李韩平鲍作义王晓林李天一

李敬云

 

军事医学科学院微生物流行病研究所 病原微生物生物安全国家重点实验室 全军艾滋病检测中心 北京100071

Author(s):
 

Wang Zheng Zhuang Dao-min Liu Si-yang Chen Li-li Li lin Liu Yong-jian Li Han-ping Bao Zuo-yi Wang Xiao-lin Li Tian-yi Li Jing-yun

 PLA center for HIV test, State Key Laboratory of pathogen and biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071.
关键词:
 HIV-1 近似全长序列 男男性行为人群
Keywords:
 

HIV-1 Near-full length sequence Men who have sex with men(MSM)

分类号:
R512.91;
DOI:
-
文献标识码:
A
摘要:
 

目的:对5名北京市男男性行为者体内HIV-1病毒全基因组特征进行全面分析。

方法:采用淋巴细胞共培养法分离出病毒,并采用RT-PCR扩增出其近似全长基因组,测序后对其基因序列特征进行深入分析。

结果:经系统进化和重组分析,分离出的5株病毒分属CRF07_BC、CRF01_AE和B亚型。病毒虽然分属不同亚型,但膜区糖基化位点的数目和分布位置都趋于保守,V3环顶端序列存在GPGR和GWGR两种形式,嗜性预测皆为M嗜性。结论:男男性行为人群中HIV-1流行毒株存在CRF01_AE、CRF07_BC和B亚型。本研究获得的全长序列将为研究HIV-1各亚型在同性恋人群中的传播和进化提供理论依据。

Abstract:
 

Object: Overall analysis of HIV-1 near-full length sequences which was isolated from MSM

Methods: Viral strains were isolated by co-culturing PBMCs from infected individuals and healthy blood donors, and the near-full length genomes were amplified by RT-PCR method, further analyses of gene characteristics were performed after sequencing.

Results: 5 isolated strains are classified as CRF01_AE, CRF07_BC, and B subtype separately. The numbers and sites of potential N-linked glycosylations within the Env are conservative despite the strains belong to the different clades, GPGR and GWGR included in the V3 region and the strains are predicted as M tropism.

Conclusion: HIV-1 clades circulating in MSM include CRF01_AE, CRF07_BC, and B. These full-length sequences will provide theoretic basis on the research of transmission and molecular evolution of HIV-1 circulating in the MSM.

参考文献/References

 

备注/Memo

备注/Memo:
更新日期/Last Update: 2011-04-15